Unilong
14 Years Production Experience
Own 2 Chemicals Plants
Passed ISO 9001:2015 Quality System

Manufacturer for 111-60-4 - Proteinase K with cas 39450-01-6 – Unilong


Product Detail

Download

Product Tags

Related Video

Feedback (2)

To continuously increase the administration process by virtue of the rule of "sincerely, good religion and excellent are the base of company development", we commonly absorb the essence of linked goods internationally, and continually build new solutions to fulfill the requires of shoppers for 305-84-0, Cosmetic Material, Polycaprolactone (Pcl) Cas24980-41-4, We have confident that we can provide the high quality products at resonable price, good after-sales service to the customers. And we will create a bright future.
Manufacturer for 111-60-4 - Proteinase K with cas 39450-01-6 – Unilong Detail:

No.1 liquid

Protein specific activity>800U/mL, the concentration is 20mg/mL, no Nickase residue

No.2 powder

Appearance White lyophilized powder
Electrophoretic purity ≥95%
Enzyme activity ≥30U/mg
Nucleic acid residue Invisible
Deoxyribonuclease residue Invisible
Ribonuclease residue Invisible
Cart No. G1205-10ML
Storage When stored at –20 °C, the product retains activity for at least 1 years, ice bag transportation.

Proteinase K is a serine protease belonging to the subtilisin family with efficient enzymatic activity and broad substrate specificity, which preferentially decomposes ester bonds and peptides adjacent to the C-terminus of hydrophobic amino acids, sulfur-containing amino acids, and aromatic amino acids bonds, which are often used to degrade proteins to produce short peptides. Proteinase K has the characteristic of the typical catalytic triad Asp39-His69-Ser224 unique to serine proteases and has two Ca2+ binding sites around the active center to increase its stability and maintain high enzymatic activity under a wider range of conditions.

1. Genetic diagnosis kit.
2. RNA and DNA extraction kits.
3. Extract non-protein components in tissues and degrade protein impurities (such as preparation of DNA vaccines and heparin).
4. Preparation of Chromosomal DNA for Pulse Electrophoresis.
5. Western blotting.
6. Development and production of enzymatic glycated albumin reagents in the field of in vitro diagnostics.

1. 30mg/bottle 1g/bottle or 100g/bottle
2. 1kgs/foil bag

Proteinase K is a stable serine protease with broad substrate specificity. It degrades many proteins in the native state even in the presence of detergents. It is not activated by metal ions, chelating agents (for example, EDTA), sulfhydryl reagents, or by tripsin or chymotrypsin inhibitors. It is stable over a wide pH range (4–12.5), with optimal activity at pH 6.5–9.5. Activity can be stimulated by addition of denaturing agents (SDS and Urea). Rapid denaturation of the enzyme occurs at temperature above 70°C. Autolysis of the enzyme occurs increasingly at alkaline pH. However, Proteinase K is not completely inactivated by autolysis. Some enzyme fragments continue to maintain their complete proteolytic activity, even after extensive autolysis. Proteinase K is frequently used in molecular biology applications to digest unwanted proteins, such as nucleases in DNA or RNA preparations from microorganisms, cultured cells, and plants. The enzyme is typically used at 50–200 ug/ml in nucleic acid preparations at pH 7.5–8.0 and 37~55oC. Incubation times vary from 30 minutes to 18 hours.

A stable and highly reactive serine protease; used for protein and nucleic acid isolation.Proteinase K is used in the purification of RNA and DNA from tissues or cell lines.


Product detail pictures:

Manufacturer for 111-60-4 - Proteinase K with cas 39450-01-6 – Unilong detail pictures

Manufacturer for 111-60-4 - Proteinase K with cas 39450-01-6 – Unilong detail pictures

Manufacturer for 111-60-4 - Proteinase K with cas 39450-01-6 – Unilong detail pictures


Related Product Guide:

With this motto in mind, we've got become among essentially the most technologically innovative, cost-efficient, and price-competitive manufacturers for Manufacturer for 111-60-4 - Proteinase K with cas 39450-01-6 – Unilong , The product will supply to all over the world, such as: India, Oslo, Orlando, Our expert engineering team will generally be prepared to serve you for consultation and feedback. We are able to also offer you with free of charge samples to meet your requirements. Best efforts will likely be produced to provide you the best service and merchandise. When you are keen on our business and products, please speak to us by sending us emails or call us quickly. In an effort to know our products and company extra, you may come to our factory to view it. We will generally welcome guests from all over the world to our business to create business relations with us. Please feel cost-free to speak to us for small business and we believe we'll share the best trading experience with all our merchants.
  • This is a very professional and honest Chinese supplier, from now on we fell in love with the Chinese manufacturing.
    5 Stars By Jacqueline from Johannesburg - 2018.06.09 12:42
    Good quality and fast delivery, it's very nice. Some products have a little bit problem, but the supplier replaced timely, overall, we are satisfied.
    5 Stars By Beulah from Buenos Aires - 2018.09.29 17:23
    Write your message here and send it to us