HEPES CAS 7365-45-9
HEPES CAS 7365-45-9 has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
ITEM | STANDARD |
Appearance | white crystalline powder |
Application | PH manager |
PH value | 5.0-6.5 (1Maqueous solution) |
Fusing point | 234-238 °C |
Boiling point | 408℃[at 101 325 Pa] |
Packing density | 560kg/m3 |
Density | 1.07 g/mL at 20 °C |
Storage condition | 2-8°C |
Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.
25kgs/drum,9tons/20'container,25kgs/bag,20tons/20'container.
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HEPES CAS 7365-45-9
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HEPES CAS 7365-45-9