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Factory Cheap Hot 4316-74-9 - Proteinase K with cas 39450-01-6 – Unilong


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Factory Cheap Hot 4316-74-9 - Proteinase K with cas 39450-01-6 – Unilong Detail:

No.1 liquid

Protein specific activity>800U/mL, the concentration is 20mg/mL, no Nickase residue

No.2 powder

Appearance White lyophilized powder
Electrophoretic purity ≥95%
Enzyme activity ≥30U/mg
Nucleic acid residue Invisible
Deoxyribonuclease residue Invisible
Ribonuclease residue Invisible
Cart No. G1205-10ML
Storage When stored at –20 °C, the product retains activity for at least 1 years, ice bag transportation.

Proteinase K is a serine protease belonging to the subtilisin family with efficient enzymatic activity and broad substrate specificity, which preferentially decomposes ester bonds and peptides adjacent to the C-terminus of hydrophobic amino acids, sulfur-containing amino acids, and aromatic amino acids bonds, which are often used to degrade proteins to produce short peptides. Proteinase K has the characteristic of the typical catalytic triad Asp39-His69-Ser224 unique to serine proteases and has two Ca2+ binding sites around the active center to increase its stability and maintain high enzymatic activity under a wider range of conditions.

1. Genetic diagnosis kit.
2. RNA and DNA extraction kits.
3. Extract non-protein components in tissues and degrade protein impurities (such as preparation of DNA vaccines and heparin).
4. Preparation of Chromosomal DNA for Pulse Electrophoresis.
5. Western blotting.
6. Development and production of enzymatic glycated albumin reagents in the field of in vitro diagnostics.

1. 30mg/bottle 1g/bottle or 100g/bottle
2. 1kgs/foil bag

Proteinase K is a stable serine protease with broad substrate specificity. It degrades many proteins in the native state even in the presence of detergents. It is not activated by metal ions, chelating agents (for example, EDTA), sulfhydryl reagents, or by tripsin or chymotrypsin inhibitors. It is stable over a wide pH range (4–12.5), with optimal activity at pH 6.5–9.5. Activity can be stimulated by addition of denaturing agents (SDS and Urea). Rapid denaturation of the enzyme occurs at temperature above 70°C. Autolysis of the enzyme occurs increasingly at alkaline pH. However, Proteinase K is not completely inactivated by autolysis. Some enzyme fragments continue to maintain their complete proteolytic activity, even after extensive autolysis. Proteinase K is frequently used in molecular biology applications to digest unwanted proteins, such as nucleases in DNA or RNA preparations from microorganisms, cultured cells, and plants. The enzyme is typically used at 50–200 ug/ml in nucleic acid preparations at pH 7.5–8.0 and 37~55oC. Incubation times vary from 30 minutes to 18 hours.

A stable and highly reactive serine protease; used for protein and nucleic acid isolation.Proteinase K is used in the purification of RNA and DNA from tissues or cell lines.


Product detail pictures:

Factory Cheap Hot 4316-74-9 - Proteinase K with cas 39450-01-6 – Unilong detail pictures

Factory Cheap Hot 4316-74-9 - Proteinase K with cas 39450-01-6 – Unilong detail pictures

Factory Cheap Hot 4316-74-9 - Proteinase K with cas 39450-01-6 – Unilong detail pictures


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With a positive and progressive attitude to customer's interest, our enterprise continually improves our products excellent to meet the wants of customers and further focuses on safety, reliability, environmental requirements, and innovation of Factory Cheap Hot 4316-74-9 - Proteinase K with cas 39450-01-6 – Unilong , The product will supply to all over the world, such as: Estonia, Puerto Rico, Eindhoven, We have got constantly insisted on the evolution of solutions, spent good funds and human resource in technological upgrading, and facilitate production improvement, meeting the wants of prospects from all countries and regions.
  • The company keeps to the operation concept "scientific management, high quality and efficiency primacy, customer supreme", we have always maintained business cooperation. Work with you,we feel easy!
    5 Stars By Rigoberto Boler from Nicaragua - 2018.05.22 12:13
    The accounts manager made a detailed introduction about the product, so that we have a comprehensive understanding of the product, and ultimately we decided to cooperate.
    5 Stars By Ricardo from Lebanon - 2018.10.31 10:02
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