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Best Price for 27905-45-9 - Proteinase K with cas 39450-01-6 – Unilong


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Best Price for 27905-45-9 - Proteinase K with cas 39450-01-6 – Unilong Detail:

No.1 liquid

Protein specific activity>800U/mL, the concentration is 20mg/mL, no Nickase residue

No.2 powder

Appearance White lyophilized powder
Electrophoretic purity ≥95%
Enzyme activity ≥30U/mg
Nucleic acid residue Invisible
Deoxyribonuclease residue Invisible
Ribonuclease residue Invisible
Cart No. G1205-10ML
Storage When stored at –20 °C, the product retains activity for at least 1 years, ice bag transportation.

Proteinase K is a serine protease belonging to the subtilisin family with efficient enzymatic activity and broad substrate specificity, which preferentially decomposes ester bonds and peptides adjacent to the C-terminus of hydrophobic amino acids, sulfur-containing amino acids, and aromatic amino acids bonds, which are often used to degrade proteins to produce short peptides. Proteinase K has the characteristic of the typical catalytic triad Asp39-His69-Ser224 unique to serine proteases and has two Ca2+ binding sites around the active center to increase its stability and maintain high enzymatic activity under a wider range of conditions.

1. Genetic diagnosis kit.
2. RNA and DNA extraction kits.
3. Extract non-protein components in tissues and degrade protein impurities (such as preparation of DNA vaccines and heparin).
4. Preparation of Chromosomal DNA for Pulse Electrophoresis.
5. Western blotting.
6. Development and production of enzymatic glycated albumin reagents in the field of in vitro diagnostics.

1. 30mg/bottle 1g/bottle or 100g/bottle
2. 1kgs/foil bag

Proteinase K is a stable serine protease with broad substrate specificity. It degrades many proteins in the native state even in the presence of detergents. It is not activated by metal ions, chelating agents (for example, EDTA), sulfhydryl reagents, or by tripsin or chymotrypsin inhibitors. It is stable over a wide pH range (4–12.5), with optimal activity at pH 6.5–9.5. Activity can be stimulated by addition of denaturing agents (SDS and Urea). Rapid denaturation of the enzyme occurs at temperature above 70°C. Autolysis of the enzyme occurs increasingly at alkaline pH. However, Proteinase K is not completely inactivated by autolysis. Some enzyme fragments continue to maintain their complete proteolytic activity, even after extensive autolysis. Proteinase K is frequently used in molecular biology applications to digest unwanted proteins, such as nucleases in DNA or RNA preparations from microorganisms, cultured cells, and plants. The enzyme is typically used at 50–200 ug/ml in nucleic acid preparations at pH 7.5–8.0 and 37~55oC. Incubation times vary from 30 minutes to 18 hours.

A stable and highly reactive serine protease; used for protein and nucleic acid isolation.Proteinase K is used in the purification of RNA and DNA from tissues or cell lines.


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Best Price for 27905-45-9 - Proteinase K with cas 39450-01-6 – Unilong detail pictures

Best Price for 27905-45-9 - Proteinase K with cas 39450-01-6 – Unilong detail pictures

Best Price for 27905-45-9 - Proteinase K with cas 39450-01-6 – Unilong detail pictures


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We have been experienced manufacturer. Wining the majority of the crucial certifications of its market for Best Price for 27905-45-9 - Proteinase K with cas 39450-01-6 – Unilong , The product will supply to all over the world, such as: Sao Paulo, Armenia, Eindhoven, We'll continue to devote ourselves to market & product development and build a well-knit service to our customer to create a more prosperous future. Please contact us today to find out how we can work together.
  • The supplier cooperation attitude is very good, encountered various problems, always willing to cooperate with us, to us as the real God.
    5 Stars By tobin from Sierra Leone - 2017.08.21 14:13
    Although we are a small company, we are also respected. Reliable quality, sincere service and good credit, we are honored to be able to work with you!
    5 Stars By Honorio from Frankfurt - 2018.09.29 17:23
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