Unilong
14 Years Production Experience
Own 2 Chemicals Plants
Passed ISO 9001:2015 Quality System

Best Price for 27905-45-9 - Proteinase K with cas 39450-01-6 – Unilong


Product Detail

Download

Product Tags

Related Video

Feedback (2)

We've been convinced that with joint efforts, the enterprise between us will bring us mutual benefits. We are able to assure you product or service quality and aggressive cost for 110615-47-9, 130120-57-9, N-Methyltaurine Sodium Salt 40%, Welcome to contact us if you are interested in our product, we will give you a surprice for Qulity and Price.
Best Price for 27905-45-9 - Proteinase K with cas 39450-01-6 – Unilong Detail:

No.1 liquid

Protein specific activity>800U/mL, the concentration is 20mg/mL, no Nickase residue

No.2 powder

Appearance White lyophilized powder
Electrophoretic purity ≥95%
Enzyme activity ≥30U/mg
Nucleic acid residue Invisible
Deoxyribonuclease residue Invisible
Ribonuclease residue Invisible
Cart No. G1205-10ML
Storage When stored at –20 °C, the product retains activity for at least 1 years, ice bag transportation.

Proteinase K is a serine protease belonging to the subtilisin family with efficient enzymatic activity and broad substrate specificity, which preferentially decomposes ester bonds and peptides adjacent to the C-terminus of hydrophobic amino acids, sulfur-containing amino acids, and aromatic amino acids bonds, which are often used to degrade proteins to produce short peptides. Proteinase K has the characteristic of the typical catalytic triad Asp39-His69-Ser224 unique to serine proteases and has two Ca2+ binding sites around the active center to increase its stability and maintain high enzymatic activity under a wider range of conditions.

1. Genetic diagnosis kit.
2. RNA and DNA extraction kits.
3. Extract non-protein components in tissues and degrade protein impurities (such as preparation of DNA vaccines and heparin).
4. Preparation of Chromosomal DNA for Pulse Electrophoresis.
5. Western blotting.
6. Development and production of enzymatic glycated albumin reagents in the field of in vitro diagnostics.

1. 30mg/bottle 1g/bottle or 100g/bottle
2. 1kgs/foil bag

Proteinase K is a stable serine protease with broad substrate specificity. It degrades many proteins in the native state even in the presence of detergents. It is not activated by metal ions, chelating agents (for example, EDTA), sulfhydryl reagents, or by tripsin or chymotrypsin inhibitors. It is stable over a wide pH range (4–12.5), with optimal activity at pH 6.5–9.5. Activity can be stimulated by addition of denaturing agents (SDS and Urea). Rapid denaturation of the enzyme occurs at temperature above 70°C. Autolysis of the enzyme occurs increasingly at alkaline pH. However, Proteinase K is not completely inactivated by autolysis. Some enzyme fragments continue to maintain their complete proteolytic activity, even after extensive autolysis. Proteinase K is frequently used in molecular biology applications to digest unwanted proteins, such as nucleases in DNA or RNA preparations from microorganisms, cultured cells, and plants. The enzyme is typically used at 50–200 ug/ml in nucleic acid preparations at pH 7.5–8.0 and 37~55oC. Incubation times vary from 30 minutes to 18 hours.

A stable and highly reactive serine protease; used for protein and nucleic acid isolation.Proteinase K is used in the purification of RNA and DNA from tissues or cell lines.


Product detail pictures:

Best Price for 27905-45-9 - Proteinase K with cas 39450-01-6 – Unilong detail pictures

Best Price for 27905-45-9 - Proteinase K with cas 39450-01-6 – Unilong detail pictures

Best Price for 27905-45-9 - Proteinase K with cas 39450-01-6 – Unilong detail pictures


Related Product Guide:

Adhering into the basic principle of "quality, assistance, effectiveness and growth", we have attained trusts and praises from domestic and worldwide client for Best Price for 27905-45-9 - Proteinase K with cas 39450-01-6 – Unilong , The product will supply to all over the world, such as: Gambia, Puerto Rico, Angola, We offer OEM services and replacement parts to meet the varying needs of our customers. We offer competitive price for quality products and we will make certain your shipment is handled quickly by our logistics department. We sincerely hope to have the opportunity to meet with you and see how we can help you further your own business.
  • This manufacturer can keep improving and perfecting products and service, it is in line with the rules of market competition, a competitive company.
    5 Stars By Ivy from Palestine - 2017.01.28 18:53
    This is a honest and trustworthy company, technology and equipment are very advanced and the prodduct is very adequate, there is no worry in the suppliment.
    5 Stars By Rachel from Peru - 2017.11.20 15:58
    Write your message here and send it to us